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recombinant proteins ru 521 invivogen  (InvivoGen)


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    InvivoGen recombinant proteins ru 521 invivogen
    Recombinant Proteins Ru 521 Invivogen, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+proteins+ru+521+invivogen/RU%2E521/pm40986423-277-31-34
    Average 95 stars, based on 39 article reviews
    recombinant proteins ru 521 invivogen - by Bioz Stars, 2026-10
    95/100 stars

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    Microarray:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Recombinant:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Transfection:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    One Step RT-PCR:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Real-time Polymerase Chain Reaction:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Ubiquitin Proteomics:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Control:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Sequencing:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..

    Single Cell RNA-Seq:

    Article Title: RSK1 reprograms the ubiquitin pathway to promote immune suppression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-GFP Santa Cruz Biotechnology Cat# sc-9996, RRID: AB_627695 anti-Ubiquitin Santa Cruz Biotechnology Cat# sc-8017 RRID: AB_628423 anti-K33-linked ubiquitin This paper N/A anti-MYC Proteintech Cat# 16286-1-AP, RRID: AB_11182162 anti-FLAG Proteintech Cat# 20543-1-AP, RRID: AB_11232216 anti-HA Proteintech Cat# 51064-2-AP, RRID: AB_11042321 anti-His Proteintech Cat# 10001-0-AP, RRID: AB_2090265 anti-ACTIN Diagbio Cat# db10001, RRID: AB_2860038 anti-mouse GAPDH Proteintech Cat# 60004-1, RRID: AB_2107436 anti-RNF19A Bethyl Laboratories Cat# A303-105A, RRID:AB_10749181 anti-RNF19A ABclonal Cat# A12036, RRID: AB_210551 anti-UBE2L6 for IHC ABclonal Cat# A4283, RRID: AB_2841480 anti-UBE2L6 for western blot ABclonal Cat# A13670, RRID: AB_2799098 anti-ISG15 ABclonal Cat# A24, RRID: AB_2126302 anti-S26p This paper N/A anti-hcGAS Cell Signaling Technology Cat# 15102S, RRID: AB_2732795 anti-mcGAS Cell Signaling Technology Cat# 31659S, RRID: AB_2799008 anti-Phospho-TBK1 Cell Signaling Technology Cat# 5483S, RRID: AB_10693472 anti-Phospho-hSTING Cell Signaling Technology Cat# 19781S, RRID: AB_2827656 anti-Phospho-mSTING Cell Signaling Technology Cat# 72971S, RRID: AB_2799831 anti-Phospho-IRF3 Cell Signaling Technology Cat# 4947S, RRID: AB_2773013 anti-STING Cell Signaling Technology Cat# 13647S, RRID: AB_2732796 anti-TBK1 Cell Signaling Technology Cat# 3013S, RRID: AB_2255663 anti-IRF3 Cell Signaling Technology Cat# 4302S, RRID: AB_2722521 anti-Rabbit IgG Abmart Cat# T55125 PerCP anti-mouse CD45 Biolegend Cat# 103130, RRID: AB_893339 APC anti-mouse CD8a Biolegend Cat# 100712, RRID: AB_312751 FITC anti-mouse CD3e Biolegend Cat# 100204, RRID: AB_312661 PE anti-mouse CD4 Biolegend Cat# 100408, RRID: AB_312693 anti-CD8α for IHC Cell Signaling Technology Cat# 85336T, RRID: AB_P01732 APC anti-mouse CD11b Biolegend Cat# 101212, RRID: AB_312795 PE anti-mouse F4/80 Biolegend Cat# 123110, RRID: AB_893486 PE anti-mouse CD11c Biolegend Cat# 117308, RRID: AB_313777 Bacterial and virus strains HSV-1 ICP8 Abcam Cat# ab20194 HSV-1 Gift from X. Wang (Zhejiang University) N/A VSV Gift from X. Wang (Zhejiang University) N/A DH5α Chemically Competent Cell Tsingke Biotechnology Cat# TSC-C14 BL21 Star (DE3) Chemically Competent Cell Tsingke Biotechnology Cat# TSC-E06 Biological samples Patient-derived colorectal cancer and paracancerous tissue This paper N/A Patient-derived breast cancer and paracancerous tissue This paper N/A (Continued on next page) Cell Reports 44, 116323, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Patient-derived colorectal cancer tissue microarray This paper N/A Patient-derived lung cancer tissue microarray This paper N/A Patient-derived Glioblastoma tissue microarray This paper N/A Chemicas, peptides, and recombinant proteins RU.521 InvivoGen Cat# inh-ru521 PMD-026 ProbeChem Cat# PC-72284 RSK-IN-1 MedChemExpress Cat# 2761698-77-3 Puromycin Dihydrochloride Yeasen Biotech Cat# 60210ES60 proteinase K Thermo Fisher EO0491 Trypsin GIBCO Cat#15400054 DMEM GIBCO Cat#C11995 2 ′ 3 ′ -cGAMP InvivoGen Cat# tlrl-nacga23 HT-DNA Sigma-Aldrich Cat# D7290 poly (I:C) InvivoGen Cat# tlrl-picwlv Cycloheximide Beyotime Cat# SC0353 MG132 Beyotime Cat# S1748 Lipofectamine 2000 Invitrogen Cat# 11668 Hieff Trans TM Liposomal Transfection Reagent Yeasen Biotech Co., Ltd. Cat# 40802ES02 Polybrene Yeasen Biotech Co., Ltd. Cat# 40804ES76 Glutathione Sepharose Yeasen Biotech Co., Ltd. Cat# 30903ES10 TRIZOL Vazyme Biotech Co.,Ltd Cat# RC201 HiScript II One Step RT-PCR Kit Vazyme Biotech Co.,Ltd Cat# P611-01 ChamQ Universal SYBR qPCR Master Mix Vazyme Biotech Co.,Ltd Cat# Q711-02 Anti-FLAG agarose Sigma-Aldrich Cat# A2220 HA beads Abmart Cat# M20013 MYC beads Abmart Cat# M20012 Protein A/G beads Santa Cruz Biotechnology Cat# sc-2003 GST-Glutathione Agarose Resin Yeasen Biotech Cat# 20507ES60 Ni NTA Beads Smart-lifesciences Cat# SA004005 Ubiquitin-Activating Enzyme E1/UBA1 Protein Sinobiological Cat# 11990-H20B Critical commercial assays InVivoPlus anti-mouse PD-1 Bio X Cell Cat# BE0146, RRID: AB_10949053 InVivoPlus rat IgG2a isotype control Bio X Cell Cat# BE0089, RRID: AB_1107769 2 ′ ,3 ′ -Cyclic GAMP ELISA Kit Arbor Assays Cat# K067-H1 Deposited data RNA sequence This paper GSE305014 Single cell RNA-seq This paper GSE305014 Raw data This paper https://doi.org/10.17632/zv4gtdtdt7.1 Experimental models: Cell lines HEK293T: Human embryonic kidney 293 cells ATCC N/A MC38: murine colon adenocarcinoma cell line ATCC N/A B16F10: murine melanoma cell line ATCC N/A LLC: murine lewis lung carcinoma cell line ATCC N/A RCC4: human kidney cancer cell lines ATCC N/A CT-2A: murine glioblastoma cell line ATCC N/A Experimental models: Organisms/strains Rnf19a− /− mice This paper N/A (Continued on next page) 18 Cell Reports 44, 116323, October 28, 2025 ..



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